AMP Certificate Program: NGS101 v2.0: A Re-Introduction to the Fundamentals of Next-Generation Sequencing

This AMP certificate program contains about 7 hours of lecture content, the associated pdf slides of the lectures, pre-tests, and post-lecture tests, and an AMP Certificate of Completion. 

CME and CMLE credits are not available for this course.

NGS101 v2.0 course is an update of the very popular NGS101. Whether you’re looking to add Next-Generation Sequencing (NGS) to your lab or need a refresher, this course delivers the most current and useful information to help you in your decision making process. This 7-part course (including 6.50 hrs of CME or CMLE credit) is a comprehensive primer on implementing and running NGS in your laboratory.

The self-paced course contains a pre-test to test your baseline knowledge of the content. It is then followed by presentations by subject matter experts and then a post-test after each section to test your knowledge of the content. Some sections will include supplementary resources and tools, such a list of links to suggested online tools and guidelines.

Showcase your knowledge by completing the entire course and passing each post-test with a score of 75% or greater and receiving the AMP Certificate of Completion.

Target Audience: Molecular pathologists, trainees, residents, laboratory directors, technologists and others who have some prior exposure to molecular diagnostic testing using NGS. Oncologists, genetic counselors, primary care clinicians, and other health care professionals who will receive NGS finding reports will also benefit from this course.

Topics Covered in Certificate Program: For a detailed outline, click here.

  1. Value of NGS in Oncology and Germline Genetic Testing
  2. Should I Consider NGS for my Laboratory?
  3. Assay Design Considerations (How to Decide What is Best for my Lab?)
  4. How to Approach Validation and Ensure Ongoing Quality
  5. I Found a Variant...Am I Finished? Variant Review (IGV, confirmation), Interpretation and Reporting
  6. What Might go Wrong?
  7. Advanced Topics, Future Directions

Learning Objectives:

  • Summarize factors that contribute to the selection and use of NGS-specific platforms in the context of individual laboratory needs, technical limitations, and reporting goals. 
  • Describe the range of molecular pathology applications for NGS data.
  • Describe considerations for the design and implementation of NGS-specific bioinformatics pipelines.
  • Implement current approaches for validation testing of NGS-based results.
  • Discuss key considerations for variant identification and classification, including the use of specific bioinformatics tools, accuracy and sensitivity, and dealing with poorly characterized variants.
  • Utilize NGS data to prioritize and predict pathogenicity of genomic variants, including correlating specific variants with disease and potential clinical outcomes.
  • Define best practices for reporting NGS findings to other clinical professionals.

Duration: ~7 hours
AMP Certificate Program Launched: April 20, 2021

AMP Certificate of Completion Credit available: 6.5 hours
Last day to complete the course: December 31, 2024

Watch an excerpt from the content director's welcome message. 



Joel A. Lefferts, PhD 
Content Director
Matthew S. Lebo, PhD
Working Group
Stephen J. Wicks, PhD
Working Group
Honey V. Reddi, PhD
Working Group/T&E Representative
Esther Babady, PhD
Nicole L. Hoppman, PhD Susan J. Hsiao, MD, PhD Valentina Nardi, MD
Thanks to all who contributed to the project including the AMP Training & Education Committee for their support and careful review.

Supported by an educational grant from Illumina . No personally-identifiable information regarding you is provided to any grant supporters. .


CME and CMLE credits are not available for this course.

Note: Members of AMP can access this webinar at no cost. Join the AMP Family!

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No digital files may be reproduced or transmitted in any form, by any means, electronic or mechanical. By purchasing a product, you agree to not share any of the course materials, including videos, downloadable slide presentations, outlines, manuscripts, etc. without explicit and written permission from AMP.