Practical Considerations for the Validation of Next-Generation Sequencing-Based Oncology Panels
This content was created in 2018, but has been reviewed and approved by the Training & Education Committee as useful, accurate, and relevant. Please note that in the time since this material was posted, there may have been additional developments, advancements, and/or more current publications in this field.
AMP Education is constantly updating our educational offerings, and will remove or replace content that is no longer accurate. Please be sure to use the search function to find related or updated material available in our catalogue at educate.amp.org. The Training and Education Committee works with AMP Education Programs to bring you the most up-to-date and cutting-edge information on molecular pathology research, applications, and training.
Description:
Validation of NGS-based testing for oncology panels can be challenging given the many steps and wide range of targeted mutations. In this presentation, Dr. Jennings will discuss the recent AMP/CAP guideline recommendations for validation and review practical ways to provide documented evidence with the clinical NGS reports.
Learning Objectives:
- Describe an error-based approach to validation and choose which sources of variation need to be assessed.
- Estimate the number of samples that are needed to confidently achieve a stated reliability.
- Use tolerance limits to define minimum QC performance criteria.
- Compare ways to assess library complexity.
Practice Guideline Available Here
Speaker: Lawrence J. Jennings, MD, PhDModerator: Antonia R. Sepulveda, MD, PhD
Duration: 1 hr
Level of Instruction: Basic
Date Recorded: April 25, 2018
Continuing Education Credit Information
CE Credit for this course has expired.Note: Join the AMP Family for discounted access to the most current educational resources!
All sales are final. No refunds will be issued.
No digital files may be reproduced or transmitted in any form, by any means, electronic or mechanical. By purchasing a product, you agree to not share any of the course materials, including videos, downloadable slide presentations, outlines, manuscripts, etc. without explicit and written permission from AMP.