This collection will give you access to the recordins course only. No continuing education credit is available.


This course includes all of the recorded presentations, course materials, and case studies from the live 2016 Molecular Pathology Outreach Course (MPOC) held on November 9, 2016. Learn at your own pace and rewatch lectures as many times as you'd like. Whether you're new to the field or need a refresher, this course is for you! Designed for pathologists, residents, laboratory directors and technologists who have little experience with molecular diagnostic testing, it is a staple offering that benefits professionals at any level in their career.

The course includes:

  • Contemporary hot topics in the clinical molecular diagnostics laboratory
  • An overview of DNA and genomics to build the molecular diagnostic "toolkit"
  • Short case presentations that demonstrate practical applications of a wide range of common & emerging molecular tools, including next generation sequencing, expression signature methodologies, & liquid biopsies
  • Molecular testing in clinical genetics, cancers, & infectious diseases


The introductory, overview lecture, "Contemporary Topics in Molecular Diagnostics," presents real-life case scenarios of how a "molecular toolkit" and techniques are used in today's molecular pathology diagnostics laboratory. The second lecture presents the fundamentals of DNA and genomics. Subsequently, case studies throughout the rest of the course describes applications of the technologies in various content areas (genetics, oncology, informatics, and hematopathology).


Duration: 5.5 hr

Level of Instruction: Basic


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Course Information
Course Date:
November 09, 2016
Course Objectives
  • Discuss how molecular markers can be useful in the diagnosis and prognosis of solid tissue tumors.
  • Compare and contrast genetic techniques for the identification of clinically-significant gene mutations and rearrangements.
  • Utilize the diverse palette of molecular testing available for the diagnosis and management of hematologic diseases.
  • Identify molecular techniques that can be useful in the detection of infectious diseases.
Click on a topic name to see details and purchase options.
Wednesday November 9, 2016
Speaker Information
Cecilia Yeung MD  [ view bio ]

Duration: 12 min


Talk 1: Invaders, Bacteria Older Than Dinosaurs, and Molecular Diagnostics

Learning Objectives:

1) Describe the two step process of the Invader assay.

2) Explain the origin and significance in quality control of background signal in this assay.

3) Select clinical scenarios in which this methodology is appropriate.


Talk 2: FAQs of Non-Template Amplification Methods

Learning Objectives:

1) Differentiate between template amplification and other amplification methods used in molecular diagnostics.

2) Appraise the various laboratory structural/organizational requirements for non-template amplification methods.

3) Contrast the specimen requirements/limitations for template versus non-template amplification methods.

Speaker Information
Annette S. Kim MD, PhD  [ view bio ]
Factor V
Activating Protein C
Non-template amplification
Invader Cleavase

Duration: 1 hr


Talk 1: JAK2 allele specific PCR (10min)

Learning Objectives:

  1. Describe the methodology of allele-specific PCR.
  2. Interpret a PCR melting curve for JAK2 p.V617F.
  3. Select appropriate follow-up testing in myeloproliferative disorders (MPDs).


Talk 2: PCR Engraftment techniques: When size does matter! (10min)

Learning Objectives:

  1. Describe the technology of sizing PCR
  2. Compare sizing PCR with other engraftment studies
  3. Illustrate the use of sizing PCR in various clinical and diagnostic settings.


Talk 3: Liquid Biopsies: Just Go With the Flow (10 min)

Learning Objectives:

  1. Appraise clinical scenarios where ddPCR is appropriate.
  2. Interpret ddPCR primary data.
  3. Recognize the importance of pre-analytic processing of the sample.


Talk 4: Nested PCR Assay (10min)

Learning Objectives:

  1. Describe the process of the Nested PCR assay and associated quality control considerations
  2. Identify the appropriate applications for this assay
  3. Explain the benefits and limitations of this methodology


Talk 5: The GenMark eSensor XT-8 Platform for Inherited and Infectious Disease Testing (5min)

Learning Objectives:

  1. Describe the technology underlying the GenMark eSensor XT-8 assays.
  2. Identify the indications for thrombophilia testing.
  3. Discuss the clinical utility of Hepatitis C genotyping.


Talk 6: The Luminex Platform for Multiplex Analysis in Inherited Disease and HLA testing (5min)

Learning Objectives:

  1. Describe Luminex Technology.
  2. Explain why Luminex technology is versatile in clinical laboratories.
  3. Discuss control design in the setting of multiplex assays.


Talk 7: FAQs for PCR related methods: (10min)

Learning Objectives:

  1. Explain the strategies used to eliminate the different sources of contamination during PCR
  2. Identify key criteria that influence primer design and performance
  3. Describe the template issues that can cause PCR failures


Speaker Information
Jason N. Rosenbaum MD  [ view bio ]
Rami Mahfouz MD, MPH  [ view bio ]
Annette S. Kim MD, PhD  [ view bio ]
Avni Santani PhD  [ view bio ]
Allison Cushman-Vokoun MD, PhD  [ view bio ]
myeloproliferative disorder
essential thrombocythemia
JAK2 pV617
polymerase chain reaction

ii) Ebola Virus Quantitation
iii) FAQs or Pros/Cons

Speaker Information
Anthony N. Snow MD  [ view bio ]
Colleen Kraft MD, MSc  [ view bio ]

i) Her2
ii) Technologies
iii) FAQs or Pros/Cons

Speaker Information
Anthony N. Snow MD  [ view bio ]
N. Sertac Kip MD, PhD  [ view bio ]

i) Microarray-Based Technologies for the Analysis of CNV in Cancer
ii) FAQs or Pros/Cons

Speaker Information
Juan C. Gomez-Gelvez MD  [ view bio ]

i) Targeted Bacterial Identification Using 16s PCR and Sequencing

ii) NOTCH1 Pyrosequencing in CLL

iii) FAQs or Pros/Cons

Speaker Information
Kevin Alby PhD  [ view bio ]
Jennifer Dunlap MD  [ view bio ]

i) Intro
ii) Bioinformatics
iii) Cases (SNVs, CNVs, Xlocations)
iv) FAQs or Pros/Cons

Speaker Information
Jeremy P. Segal MD, PhD  [ view bio ]
Eric J. Duncavage MD  [ view bio ]
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