This product includes the webinar and SAM credit


Recent advances in long-read sequencing technologies allows for a more complete identification of DNA and RNA associated with human disease. Two talks will be given to describe a few applications of these technologies.

Dr. Brooks will describe nanopore sequencing of full-length cDNA from chronic lymphocytic leukemia (CLL) with and without mutation in a splicing factor, SF3B1. SF3B1 is one of the most frequently mutated genes in and is associated with poor patient prognosis. While alternative splicing patterns caused by mutations in SF3B1 using short-read sequencing are known, identifying the full-length isoform changes may better elucidate the functional consequences of these mutations. The talk will describe a computational workflow for transcript analysis called FLAIR (Full-Length Alternative Isoform analysis of RNA), novel findings, and remaining challenges with long-read sequencing approaches for alternative splicing analysis.

Dr. Bloom will talk about single-molecule real time sequencing of influenza. Infection by influenza virus can activate innate immune pathways that help control viral infection. However, these pathways are only rarely activated in single virus-infected cells. This webcast will describe how combining long-read sequencing with other single-cell techniques to determine how mutations in influenza virions affect whether infected cells activate innate immune pathways.


Speakers: Angela N. Brooks, PhD and Jesse D. Bloom, PhD

Moderator: Cecilia S. Yeung, MD

Duration: 1 hr

Level of Instruction: Basic

Last day to purchase course and claim credit: March 20, 2020

Maximum SAM credit available: 1.0

How to claim credit: To earn SAM credit, all learners must watch the webinar, achieve a minimum score of 80% on the online quiz, and complete an online survey. To access the quiz, click "Submit credit" on the course homepage or click on "My Credit" on the menu to the left and then "submit credit" for the appropriate course.

This course is approved by the American Board of Pathology for 1.0 SAM credits.Physicians should only claim credit commensurate with the extent of their participation in the activity. Participants must successfully complete the online modular exams (answering at least 80% of the questions in a topic module correctly).

SAM questions edited by: Alan Brown, MD and Kristin Karner, MD

* You may not submit SAMs and CME/CMLE credit for the same content.

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Course Information
Course Date:
August 06, 2019
Course Objectives
  • Describe long-read sequencing technology for both bulk and single-cell applications
  • Describe application of long-read technology for cancer transcriptomics and to understand influenza infection
  • Describe benefits and challenges of long-read sequencing
Applications of Long-read Sequencing Technology for Cancer Transcriptomics and to Understand Influenza Infection
Individual topic purchase: Selected
American Board of Pathology
Self-Assessment Module: 1.00
Brooks & Bloom Presentation + SAM
AMP Regular Member: $0.00
AMP Technologist Member: $0.00
AMP Associate Member: $0.00
Non-member Price: $215.00