This product includes the webinar and CME/CMLE credit


Recent advances in long-read sequencing technologies allows for a more complete identification of DNA and RNA associated with human disease. Two talks will be given to describe a few applications of these technologies.

Dr. Brooks will describe nanopore sequencing of full-length cDNA from chronic lymphocytic leukemia (CLL) with and without mutation in a splicing factor, SF3B1. SF3B1 is one of the most frequently mutated genes in and is associated with poor patient prognosis. While alternative splicing patterns caused by mutations in SF3B1 using short-read sequencing are known, identifying the full-length isoform changes may better elucidate the functional consequences of these mutations. The talk will describe a computational workflow for transcript analysis called FLAIR (Full-Length Alternative Isoform analysis of RNA), novel findings, and remaining challenges with long-read sequencing approaches for alternative splicing analysis.

Dr. Bloom will talk about single-molecule real time sequencing of influenza. Infection by influenza virus can activate innate immune pathways that help control viral infection. However, these pathways are only rarely activated in single virus-infected cells. This webcast will describe how combining long-read sequencing with other single-cell techniques to determine how mutations in influenza virions affect whether infected cells activate innate immune pathways.


Speakers: Angela N. Brooks, PhD and Jesse D. Bloom, PhD

Moderator: Cecilia S. Yeung, MD

Duration: 1 hr

Level of Instruction: Basic

Last day to purchase course and claim credit: March 20, 2020

Maximum CME/CMLE credit available: 1.0

How to claim credit: To earn CME/CMLE credit, all learners must watch the webinar and then complete the online survey. To access the online survey, click "Submit credit" on the course homepage, or click on "My Credit" on the menu to the left.

AMA PRA Category 1 Credit(s)™

This activity has been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education through the joint providership of American Society for Clinical Pathology (ASCP) and Association for Molecular Pathology (AMP). The American Society for Clinical Pathology (ASCP) is accredited by the ACCME to provide continuing medical education for physicians.

The ASCP designates this enduring material for a maximum of 1.0 AMA PRA Category 1 Credit(s)™. Physicians should claim only credit commensurate with the extent of their participation in the activity.

This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1.0 hours of CMLE credit. ASCP CMLE credit hours are acceptable for the ASCP Board of Certification (BOC) Certification Maintenance Program (CMP). CMLE credit hours meet the continuing education requirements for the ASCP Board of Certification Credential Maintenance Program (CMP) and state relicensure requirements for laboratory personnel. Participants should claim only the credit commensurate with the extent of their participation in the activity. 

* You may not submit SAMs and CME/CMLE credit for the same content.

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Course Information
Course Date:
August 06, 2019
Course Objectives
  • Describe long-read sequencing technology for both bulk and single-cell applications
  • Describe application of long-read technology for cancer transcriptomics and to understand influenza infection
  • Describe benefits and challenges of long-read sequencing
Applications of Long-read Sequencing Technology for Cancer Transcriptomics and to Understand Influenza Infection
Individual topic purchase: Selected
American Medical Association
Continuing Medical Education: 1.00
American Society for Clinical Pathology
CMLE: 1.00
Brooks & Bloom Presentation + CME/CMLE
AMP Regular Member: $0.00
AMP Technologist Member: $0.00
AMP Associate Member: $0.00
Non-member Price: $205.00