This online course includes all the recorded presentations, course materials, and case studies from the live 2018 Molecular Pathology Outreach Course held on October 31, 2018 in San Antonio, TX.

CME/CMLE and SAMs credit are available with the purchase of this course.

This course is designed to provide an overview of techniques and instrumentation used from the framework of the "Molecular Pathology/Molecular Diagnostic Toolkit." In this course, faculty members will present case studies to demonstrate the toolkit’s methodologies and technologies. Emphasis will be placed on molecular testing in the areas of clinical genetics, solid tumors, hematopathology, and infectious diseases. The application of novel technologies, such as next generation sequencing and informatics, will also be highlighted.

Visit the Program page for more information.


Audience: Pathologists, residents, laboratory directors and technologists who have little experience with molecular diagnostic testing.

Duration: 3.5 hrs

Maximum CME/CMLE credit available: 3.0 

Maximum SAM credit available: 3.0 

Last day to purchase course and claim credit: October 31, 2019

Note - registrants who attended the face-to-face course do not need to register for the online course to access the online materials.

CME/CMLE and SAM Credit:

How to claim credit: To earn credit, all learners must watch the lectures, achieve a minimum score of 80% on the online quiz, and complete an online survey. To access the quiz and the online survey, click "Submit credit" on the course homepage or click on "My Credit" on the menu to the left and then "submit credit" for the appropriate course.


This activity was planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education through the joint providership of American Society for Clinical Pathology (ASCP) and the Association for Molecular Pathology (AMP). The ASCP is accredited by the ACCME to provide continuing medical education for physicians.

The ASCP designates this online educational activity for a maximum of 3.0 AMA PRA Category 1 Credit(s)TM. Physicians should claim only the credit commensurate with the extent of their participation in the activity.

This continuing medical laboratory education activity is recognized by the ASCP for up to 3.0 hours of CMLE credit. ASCP CMLE credit hours meet the continuing education requirements for the ASCP Board of Certification Credential Maintenance Program (CMP) and state relicensure requirements for laboratory personnel. Participants should claim only the credit commensurate with the extent of their participation in the activity.

SAM Credit

This activity is approved by the American Board of Pathology for a maximum of 3.0 SAM Credits. Physicians should only claim credit commensurate with the extent of their participation in the activity. Participants must successfully complete the online exam (answering at least 80% of the questions correctly).

SAM edited by: Yi Ding, MD, PhD; Honey Reddi, PhD; Midhat Farooqi, PhD

* You may not submit SAMs and CME/CMLE credit for the same content.

All sales are final. No refunds will be issued.

No digital files may be reproduced or transmitted in any form, by any means, electronic or mechanical. By purchasing a product, you agree to not share any of the course materials, including videos, downloadable slide presentations, outlines, manuscripts, etc. without explicit and written permission from AMP.

Course Information
Course Date:
October 31, 2018
Course Objectives
  • Compare and contrast a number of commonly utilized "ready out of the box" platforms with regard to their non-template amplification methodology and their clinical utilization
  • Enumerate several technological and clinical adaptations for PCR, non-PCR based amplification and array platforms
  • Discuss the methodologic and clinical considerations of when to implement testing by NGS
  • Compare and contrast methods of single nucleotide variant detection, copy number detection, and structural variant (fusions) detection by NGS and other related platforms (for CNVs).
Welcome and Introductions
HOT TOPICS in Molecular Diagnostics

Speaker: Kojo S. J. Elenitoba-Johnson, MD, University of Pennsylvania, Perelman School of Medicine

Molecular Testing Landscape — Pre-analytical Considerations

Learning Objectives

  • Compare main types of samples submitted for molecular testing highlighting key criteria for specimen selection.
  • Determine the rationale for specimen requirements for molecular testing.
  • Assess requirements for the sample adequacy in relation to the test performance characteristics.

Speaker: Anna Yemelyanova, MD, University of Alabama at Birmingham

Signal Amplification-based Methodologies & Clinical Applications

Learning Objectives:

  • Explain the mechanism of the cleavase probe signal amplification methodology.
  • Describe the differences between signal amplification and target amplication and identify a clinical application of the methodology.

Speaker: Cynthia L. Jackson, PhD, Rhode Island Hospital and Brown University Alpert Medical School

Non-PCR Case Studies and Break-out Session

Case Study TitleHybridization based nucleic acid counting

Learning Objectives:

  • Recognize the utility of molecular testing in breast cancer prognosis.
  • Identify and appraise the characteristics of the NanoString Prosigna assay.
  • Identify and select the appropriate tissue specimens for use with the Prosigna assay.

SpeakerRashmi Goswami, MD PhD, University of Toronto

PCR Methodologies and Specialized PCR Applications

Learning Objectives: 

  • Assess which method of detecting PCR amplification is most suitable for a given clinical assay.
  • Describe clinical situations which call for a single amplicon versus multiplex PCR design.

Speaker: Jeffrey Gagan, MD PhD, UT Southwestern University

PCR Case Studies and Break-out Session

Case Study Title: Nested Multiplex PCR for Multi-Pathogen Meningitis Detection

Learning Objectives:

  • Describe the clinical utility of multiplex syndromic panels.
  • Explain the advantages and limitations of the meningitis syndromic panel testing.

SpeakerPreeti Pancholi, PhD, Ohio State University

Micro Array and Hybridization-based Technology and its Clinical Application

Learning Objectives:

  • Describe microarray technology.
  • Apply microarray technology.

Speaker: Yassmine Akkari, PhD, Legacy Health

Array Case Studies and Break-out Session

Title of Case Study: Microarrays in Oncology:  Case Studies

Learning Objective:

  • Apply microarray technology in oncology case studies.

SpeakerYassmine Akkari, PhD, Legacy Health

Introduction to NGS Platform & Clinical Applications AND Introduction to Bioinformatics Pipeline & Data Analysis

Title: Introduction to NGS Platform & Clinical Applications

Learning Objectives:

  • Describe the two main steps of a library preparation and some of the different variations that are possible.
  • Compare different next-generation sequencing platforms and understand the basic chemical differences.
  • Provide examples of clinical applications for NGS.

Speaker: Cecilia C. S. Yeung, MD, Fred Hutchinson Cancer Research Center


Title: Introduction to Bioinformatics Pipeline & Data Analysis

Learning Objectives:

  • Select the appropriate database to research a given genomic variant.
  • Arrange the essential steps of a DNA next-generation sequencing bioinformatics pipeline.

Speaker: Joshua Coleman, MD, University of Utah

NGS Case Studies and Break-out Session

Title of Case StudyUsing Next Generation Sequencing in Constitutional Genetics: Glycogen Storage Disease

Learning Objectives:

  • Discuss indications, diagnosis, and typing of glycogen storage disease and the benefits of using next generation sequencing.
  • Describe NGS sequencing and analysis in constitutional genetics using the Ion Torrent PGM.
  • Discuss criteria for identification and reporting of variants found. 

SpeakerBarbara Anderson, BS, MS, Duke Molecular Diagnostic Laboratory

Wrap up and Additional Materials
Individual topic purchase: Selected
American Board of Pathology
Self-Assessment Module: 3.00
American Medical Association
Continuing Medical Education: 3.00
American Society for Clinical Pathology
CMLE: 3.00
This seminar is no longer available for purchase.